基于蛋白A法的禽流感压电免疫芯片的研究

A Piezoelectric Immunosensor for Avian Influenza(AIV) Viruses by SPA Method

目的:研制基于蛋白A法的禽流感压电免疫芯片。方法:以AT切型、基频10MHz的石英晶体为材料,设计压电免疫芯片。采用蛋白A法将禽流感H5亚型单克隆抗体和抗原的反应液固定在石英晶体的金膜电极表面,构建可用于禽流感检测的压电免疫芯片。结果:蛋白A法若先固定抗体,将无法检测到抗原;但可先进行抗原抗体反应再与蛋白A结合以达到定性检测的效果。结论:蛋白A法可作为禽流感压电免疫芯片抗体固定的方法,但在应用方面存在一定的局限性。

Objective： To establish a piezoelectric immunosensor for avian influenza viruses by SPA method Methods：The mixture of H5 subtype AIV monoclonal antibody and antigen is deposited on the gold electrode＇s surface of 10 MHz quartz AT-cut crystal by SPA method to made the chips. Results： If the monoclonal antibody is immobilized to SPA, the antigen could not be detected; but the mixture of H5 subtype AIV monoclonal antibody and antigen can be detected and could be used to qualitative detection. Conclusions： The SPA method could be used to establish a piezoelectric immunosensor for avian influenza viruses, but there is limitation in use.