小麦抗条锈病基因YrZH84的RGAP标记及其应用

Mapping of Wheat Stripe Rust Resistance Gene YrZH84 with RGAP Markers and Its Application

利用RGAP标记及基于标记基因型和表型选择的分离群体分组分析法,对小麦抗条锈病骨干亲本周8425B和感病品种中国春及其F2分离群体进行分析,获得了与抗条锈基因YrZH84紧密连锁的RGAP标记Xrga-1,连锁距离为0.8cM。其RGA片段长度为343bp,BLAST分析结果表明,该RGA序列与已克隆的大麦抗秆锈病基因Rpg1核苷酸序列同源性为93%,与大麦抗白粉病基因Mla家族的核苷酸序列同源性为92%。用标记Xrga-1对黄淮麦区58个小麦品种(系)进行了检测,结合系谱分析和抗病性鉴定结果,发现周8425B的衍生品种周麦11、周麦17、周麦20、周麦22、矮抗58、04中36、源育3号、05中37、宛抗18和豫展10号携带抗条锈病基因YrZH84。这些研究结果对小麦抗条锈病分子育种和YrZH84基因克隆有重要作用。

Zhou 8425B, with an effective stripe rust resistance gene YrZH84, is a hall-mark parent line of wheat （Triticum aestivum L.） in the Yellow-Huai Rivers wheat growing region. An F2 population derived from the cross Zhou 8425B/Chinese Spring and their parents were used for polymorphism analysis employing the resistance gene-analog polymorphism （RGAP） markers and bulked segregant analysis （BSA） based on marker genotypes and phenotypes selection. An RGAP marker Xrga-1 was identified, which was tightly linked to the stripe rust resistance gene YrZH84, with a genetic distance of 0.8 cM. The fragment amplified by Xrga-1 was 343 bp. BLAST analysis indicated that the rga-1 showed 93% of identical nucleotide sequences to barley stem rust resistance gene Rpg1, and 92% to barley powdery mildew resistance gene Mla homology family. Fifty-eight cultivars from Yel-low-Huai rivers wheat growing region were genotyped by the RGAP marker Xrga-1. From the information of genotypes, pedi-grees and resistance responses, the cultivars Zhoumai 11, Zhoumai 20, Zhoumai 22, Aikang 58, 04 Zhong 36, Yuanyu 3, 05 Zhong 37, Wankang 18, and Yuzhan 10 were identified to carry the resistance gene YrZH84. These cultivars were the derivatives of Zhou 8425B. These results will greatly benefit the marker-assisted selection in wheat and cloning of the resistance gene YrZH84.