摘要
取新鲜鸡肠黏膜超声波破碎,乙酸浸提,经Sephadex G-50层析后,收集具有抑菌活性的组分进行超滤离心,抑菌实验表明滤过物具有抑菌活性。滤过物经SDS-PAGE分析为一个条带,分子量约为6.078KD,得到电泳纯的鸡肠道抗微生物肽。该肽具有一定的耐热性,但经90℃以上热处理后活性下降较大;pH值对其活性影响较大,在pH6～8时活性最强。
After the intestinal mucous membranes of chicken were treated by ultrasonic treatment and acid extraction,an antimicrobial peptide was isolated from the intestine of chicken by Sephadex G-50 gel filtration chromatography and centrifugal uhrafiltration.The filtration material was analyzed by SDS-PAGE and the result showed that an antimicrobial peptide was purified and its molecule weight was about 6.078kD.The antimicrobial peptide was heat-resistant, but its activity descended obviously after heated at 90℃.The activity of the antimicrobial peptide was affected by pH and it was the strongest at pH6-8.
出处
《家禽科学》
2009年第7期3-6,共4页
Poultry Science
基金
国家"863"计划(2006AA10Z320)
安徽省优秀青年基金(04041042)
关键词
鸡
肠黏膜
抗微生物肽
温度
PH值
chicken
intestinal mucous membrane
antimicrobial peptide
temperature
pH value
