高效石油降解菌的筛选及初步鉴定

Isolation and Preliminary Identification of Oil-Degrading Microorganism

经过以石油烃为唯一碳源的选择性培养基平板初筛和三角瓶发酵复筛,采用紫外分光光度法测定石油降解率,从江汉油田和冀东油田石油污染的土壤和水体中,筛选出有降解石油能力的微生物9株。其中3株细菌(X-1,X-2,X-3)降解石油能力较高,X-1菌株的石油降解率最高达64.28%。根据形态学观察和部分生理生化特征初步鉴定,该菌为节杆菌属(Arthrobacter sp.)。

Nine strains which could degrade crude oil efficiently were isolated from oil-containing soil or water of Jianghan Oil Field and Jidong Oil Field,and were screened on the plates and flasks respectively in the selective medium with oil as sole carbon source. The results showed that three bacteria （X-1,X-2,X-3） had rela- tively high capacity of oil degradation and the best one was strain X-l, with degradation rate of oil as high as 64.28 %. It was belong to Arthrobacter sp. by preliminary identification through configuration observation and physiological and biochemical characteristics.