软脉灵对自发性高血压大鼠心肌纤维化的作用

Effect of Ruanmailing on Myocardial Remodeling in Spontaneously Hypertensive Rats

目的观察软脉灵对自发性高血压大鼠(SHR)心肌纤维化的影响。方法选择12周龄雄性SHR36只,随机分为4组,软脉灵大剂量和小剂量组、空白组、阳性对照组。血压正常的京都Wistar大鼠(WKY)为正常对照组(WKY组)。治疗前及治疗12周后用尾袖法测定动脉血压,称重后处死,取心脏,计算左心室重量与体重比(LVM/BW),天狼星红染色,计算心肌胶原容积分数。结果软脉灵大剂量、小剂量组治疗12周后,收缩压(SBP)均显著低于空白组(P<0.01)。与空白组相比,软脉灵大剂量组对SHR心肌外斜层纤维化有明显抑制作用(P<0.01),而软脉灵小剂量组则无影响;与空白组相比,软脉灵大剂量和小剂量组对SHR心肌中环层纤维化及心肌血管周围纤维化均有明显抑制作用(P<0.01)。结论软脉灵可以抑制SHR血压的发展,抑制SHR的心肌纤维化。

Objective To observe the effects of Ruanmailing （RML） on the remodeling of myocardium in spontaneously hypertensive rats （SHR）. Methods Thirty - six male SHRs were randomized into 4 groups： High - dose RML group [RH： 3 mL/（kg ·d）], low-dose RML group [RL： 1 mL/（kg · d）], untreated control （SHR）,losartan positive control [（Los： 10 mg/（kg · d）]. Systolic blood pressure was examined by the tail - cuff at 12 weeks. The ratio of wet weight of left ventricle to body weight （LVM/BW） was assessed. Myocardial collagen fibers in the sections were observed under light microscope. Results After 12 weeks treatment,systolic blood pressure in both RMI. groups was markedly lower than that in SHR （P〈0.01）. The perivascular myocardial collagen volume fraction was significantly reduced in treatment group with RH （P〈0.01）, while no significant difference in RL was found. The middlelevel of myocardial collagen volume fraction was markedly decreased in RML groups （P〈0.01）. After 12 weeks treatment with RH and RL,the artery of myocardial collagen volume fraction in both groups was significantly decreased than that in SHR（P〈0.01）. Conclusion RML might prevent the development of hypertension in SHR. RML might inhibit accumulation of cardiac collagen fibers