二苯乙烯苷在高血脂模型大鼠体内的药动学研究与组织分布

Studies on pharmacokinetics and tissue distribution of stilbene glycoside in the hyperlipidemia model rats

目的:比较研究二苯乙烯苷在正常大鼠,食靡性高脂血症模型大鼠和Triton致高脂血症模型大鼠体内的药动学及组织分布情况。方法:采用C18色谱柱(4.6mm×250mm,5μm),流速为1.0mL·min-1,检测波长320nm。测定血浆药物浓度时,以虎杖苷为内标,乙腈-甲醇-0.1%醋酸(12∶10∶78)为流动相;采用外标法测定各组织中药物浓度,乙腈-甲醇-0.1%醋酸(15∶18∶67)为流动相。结果:二苯乙烯苷在血浆和组织中的线性范围分别为0.13～81.00μg·mL-1和0.65～162.00μg·mL-1。精密度和回收率均符合生物样品分析要求。与正常大鼠相比,二苯乙烯苷在高血脂模型动物体内的药动学及组织分布发生了明显变化,结论:本方法简便,快速,灵敏度高,可用于二苯乙烯苷在高血脂模型动物体内的药动学及组织分布研究。

Objective：To comparative study the characteristics of pharmacokinetics and tissue distribution of stilbene glycoside in the normal rats,the hyperlipidemia model rats induced by feeding with the high -lipid diet and the hyperlipidemia model rats induced by intraperitoneal injection of Triton. Method：The C18 column（250 mm × 4.6 mm,5 μm） was adopted at a flow rate of 1.0 mL · min^-1. The UV detector was set at 320 nm. Polydatin was taked as the internal standard with the mobile phase consisting of acetonitrile -methanol -0. 1% acetic acid （12： 10： 78） to determine the concentrations of stilbene glycoside in plasma samples. External standard method was selected with the mobile phase consisting of acetonitrile- methanol- 0. 1% acetic acid（ 15： 18： 67）to determine the concentrations of stilbene glycoside in tissues samples. Results：The calibration curves in plasma and tissues were linear over the range of 0. 13 -81.00 μg·mL^-1 and 0. 65 - 162. 00 μg·mL^-1 , respectively. The precisions and recoveries of stilbene glycoside were corresponded to the requirement of determination for biological specimen. The characteristics of pharmacokinetics and tissue distribution of stilbene glycoside in the hyperlipidemia model rats had taken place marked change compared with the normal rats. Conclusion：The method is simple, rapid, sensitive and suitable for pharmacokinetics and tissue distribution studies of stilbene glycoside in the hyperlipidemia model rats.