毛细管气相色谱法对百树真油中多种成分的含量测定

Determination of different compound in Baishuzhen Oil by capillary GC

目的:测定百树真油中桉油精、薄荷脑、异龙脑、龙脑、桂皮醛、丁香酚的含量;方法:采用气相色谱法,色谱柱为PEG-20M毛细管柱30m×0.25mm×0.25μm,程序升温,起始温度80℃,每分钟升8℃,升到170℃后每分钟升35℃,终止温度210℃,保持4min,分流比50∶1,FID检测器;结果:桉油精的线性范围0.203～20.285mg·mL-1,薄荷脑的线性范围0.202～20.200mg·mL-1,异龙脑的线性范围0.102～10.215mg·mL-1,龙脑的线性范围0.102～10.210mg·mL-1,桂皮醛的线性范围0.132～13.190mg·mL-1,丁香酚的线性范围0.198～19.850mg·mL-1,RSD分别为1.81%,1.82%,1.23%,1.25%,1.71%,1.32%;结论:方法简便、快速、准确,可用于百树真油的质量控制。

Objective ： The purpose is to develop GC method for the assay of Eucalyptol, Menthol, Isoborneol, Borneol, Cinnamia aldehyde and Eugenol in Baishuzhen Oil. Metbods：GC was applied for quantitative analysis. Chromatographic conditions were ： PEG - 20M （30 m × 0. 25 mm × 0. 25 μm） ; programmed ; temperature ;initiation temperature at 80 ℃ ;the rate of temperature is 8 ℃/minute;the raised temperature to temperature：170 ℃ at the rate of temperature of 35 ℃/minute keep the final temperature ：210 ℃ for 4 min,bypass rate is 50： 1, Flameionization detector（FID）. Results ：The standards curve of Euealyptol, Menthol, Isoborneol, Borneol, Cinnamia aldehyde and Eugenol are linear within the range of 0. 203 -20. 285 mg·mL^-1 ,0. 202 -20. 200 mg ·mL^-1 ,0. 102 - 10. 215 mg ·mL^-1,0. 102- 10.210 mg ·mL^-1,0. 132- 13. 190 mg ·mL^-1,0. 198- 19.850 mg ·mL^-1, and RSD was 1.81% , 1.82% , 1.23% , 1.25%, 1.71% , 1.32%. Conclusion： This method is simple and accurate, and can be used for control of Baishuzhen Oil.