微柱凝胶免疫检测卡在临床输血中的应用

Clinical application of Micro-gel reagent cards in blood transfusion

目的:应用两种微柱凝胶免疫检测卡在不规则抗体筛查、交叉配血试验和直接抗人球蛋白试验中观察两者间的差异性及与传统试管法作比较。方法:应用Diamed和Biovue两种免疫检测卡对本院住院患者的血标本进行上述对比试验,凝聚胺试剂试管法进行交叉配血试验。结果:显示两种免疫检测卡做不规则抗体筛查、交叉配血试验和直接抗人球蛋白试验的符合率分别为100%、100%、99.7%,符合率完全相同。结论:两种免疫检测卡之间虽然存在着部分差异,但都可以捕捉到十分微弱的抗原抗体反应且符合率相同,比手工操作简便、标准化、易于保存,对临床安全输血有极大的帮助。

Objective： To compare the difference of microcolumn gel-direct antiglobulin test, microcolumn gel-unexpected antibody screening and microcolumn gel-crossmatch test by two kinds of microcolumn gel reagent cards and compare the test results by the traditional method. Methods： Identifing the serum of inpatients and outpatients in our hospitial to compare results of microco｜umn gel-direct antiglobulin test, unexpected antibody screening and crossmatch test by Diamed Coombs anti-IgG cards and Bi0vue Coombs anti-IgG cards. Crossmatch test bythe manual method of Polybrene reagent was perforted. Rusults： The unexpected antibody screening test, the crossmatch test and the direct antiglobulin test were done by two kinds of microcolumn gel cards. The consistent rate was 100 %, 100 ~, 99.7 ~ respectively;the difference between the results of two kinds of cards was not signifinant. Conclusions.. The results of two kinds of reagent cards have some difference, but both of two methods can detecte the lower reactions of antigens to antibodies. The consistent rate of this two kinds of cards is same. Compared with the manual method, mocrocolumn gel immuno-assay is simple, convenient, standard and easily conserved. The value of this microcolumn gel cards is important in the safety of clinical application, of blood transfusion