水蛭和虻虫不同剂量配伍对血瘀大鼠血液流变学指标的影响

Effect of different combinations of Hirudo and Tabanus on hemorheological parameters in rat model of blood stasis syndrome

目的:观察水蛭和虻虫不同剂量配伍对血瘀大鼠血液流变学的影响,探讨药对剂量配比的合理性。方法:将大鼠随机分为空白对照组、模型对照组和4个不同剂量比的水蛭、虻虫配伍组(水蛭∶虻虫分别为3∶4,4∶3,3∶1,6∶1,总剂量均为1 200 mg.kg-1)。连续给药7 d。于d 6给药1 h后,除空白对照组外,其余各组以皮下注射盐酸肾上腺素及冰水浴复制大鼠血瘀证模型。次晨给药后2 h采血,测定血液流变学指标。结果:不同配比的水蛭、虻虫药对均能明显改善大鼠血瘀模型的血液流变学异常,其中配伍2组(4∶3)、配伍3组(3∶1)对血细胞比容、红细胞聚集指数的作用较强。配伍3组(3∶1)对血浆黏度、卡松黏度的作用较强。配伍3组(3∶1)、配伍4组(6∶1)对血全黏度和全血还原黏度作用较强;配伍4组(6∶1)对红细胞变形指数作用较强。结论:在总剂量相同的条件下,水蛭剂量相对较大的配伍组(3∶1,6∶1)对血液流变学指标的作用较明显。

Objective ：To observe hemorheological effect of the different combinations of Hirudo and Taba- nus on rat models of blood stasis syndrome, and explore the rationality of different ratio of Hirudo to Tabanus. Methods：Rats were divided randomly into 6 groups： blank control group, model control group, Hirudo and Tabanus combination groups （ ratios of Hirudo to Tabanus were 3： 4,4：3,3 ： 1,6： 1, respectively, and their total dosage was 1 200mg·kg^-1 ）. The Hirudo and Tabanus were administrated by gastric gavage, qd, for 7 consecutive days. Rat models of blood stasis syndrome were established by subcutaneous injection of adrenaline combined with the stimulation of ice water. Results ： Different ratios of Hirudo to Tabanus markedly improved hemorheological parameters on rat models of blood stasis syndrome. The group with the combination proportion of 4：3 and 3：1 showed a better effect on hematocrit and erythrocyte aggregation index. The group with the combination proportion of 3：1 showed a better effect on plasma viscosity and casson viscosity. The group with the combination proportion of 3：1 and 6： I showed a better effect on blood viscosity, blood reduced viscosity. The group with the combination proportion 6：1 showed better effect on erythrocyte deformation index. Conclusion：In the different combinations groups, the group with the combination proportion of 6：1 and 3：1 showed better effect.