不同浓度镍胁迫对黄瓜种子萌发及其生理生化特性的影响

Cucumber Seed Germination and Physiological and Biochemical Characteristics Responsed to Different Concentrations of Nickel Stress

以黄瓜为材料,研究了不同浓度镍(Ni2+)胁迫对黄瓜种子萌发及黄瓜胚轴超氧化物岐化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的影响。结果表明:Ni2+浸种处理对黄瓜种子萌发具有低浓度下的激活效应和高浓度下的抑制效应,且随浓度的增加这种抑制效应逐渐增强;细胞内MDA含量在低Ni2+浓度处理下增幅较小,而后随着镍浓度的提高MDA含量逐渐增大;体内保护酶系统中SOD、CAT活性的变化表现为随Ni2+处理浓度的增加而呈现先升后降的趋势。而POD活性随着处理浓度的增加而持续升高。

Cucumbers were used to study the effect of seed germination and the activity of hypocotyls superoxide dismutase （SOD）, peroxidase （POD）, catalase （CAT） and the content of malondialdehyde （MDA） responding to different concentrations of nickel （Ni^2＋） stress. The results indicated that： Ni^2＋ treatment had activative effect under low concentrations but inhibitory effect under high concentration for cucumber seed germination, and the inhibitory effect gradually increased with the increasing concentration; the content of MDA in cells increased slightly at low concentrations of Ni^2＋, and then increased gradually as the concentration of nickel increased; the activity of SOD and CAT in the endogenous protective enzyme system increased first and then reduced with the increasing concentration of Ni^2＋. However, the activity of POD keeped increasing with the increased concentration of Ni^2＋.