高温对高羊茅几个生理指标的影响

Effects of High Temperature Stress on Several Physiochemical Indexes of Tall Fescue

研究高羊茅"猎狗5号"(Houndog5)在适温(15℃、25℃)和高温(35℃、45℃)下相对含水量(RWC)、过氧化氢酶(CAT)活性、丙二醛(MDA)含量、脯氨酸(PRO)含量等生理指标的变化。结果表明:在高温下,以15℃为对照,RWC含量和CAT活性明显下降,MDA和PRO含量明显增加,导致细胞膜脂过氧化,膜结构被破坏,生理功能紊乱,抗性下降,这是其越夏困难、易发病的主要原因。相关分析表明,RWC与CAT呈极显著正相关(r>0.95),RWC、CAT与MDA、PRO呈极显著负相关(r>-0.95),MDA与PRO呈极显著相关(r>0.95)。可选择测定相对容易的RWC和PRO两个生理指标作为选育耐热品种的重要参数,将大大减少工作量,提高工作效率。

Studied the changes of physiological Indexes ,such as RWC（relative water content）, CAT activity, MDA content,PRO content,of tall rescue ＂Houndog5＂ under the optimum temperature （15 ℃, 25℃） and high temperature （35 ℃, 45 ℃）. Compared with the temperature of 15 ℃, the results showed that the RWC and the CAT activity decreased significantly, MDA and PRO content increased obviously under high temperatur,then cell membrane-lipid peroxided,membrane structure be destroyed,physiologic function disordered and resistance decreased,these were main reasons of tall fescue oversummering hard and get ill easily.The correlation analysis showed that RWC and CAT had significant positive correlation（r〉0.95）, RWC, CAT and MDA, PRO had significant impositively correlation（r〉-0.95）, MDA and PRO had significant positively correlation（r〉 0.95）. Therefore,to select RWC and PRO determined easy as important parameters of breeding heat resistant va- riety will reduce lots of workload and improve work efficiency.