有机肥对不同发育时期烤烟叶片类胡萝卜素含量和脂氧合酶(LOX)活性的调控效应

Effect of Organic N on the Carotenoids Metabolism and Relating Enzyme Activity of Growing Flue-cured Tobacco Leaf

采用盆栽方法研究了有机肥对不同发育时期烤烟叶片类胡萝卜素含量和LOX活性的调控效应。结果表明:有机肥条件下总类胡萝卜素整个生育期含量较高;有机肥对新黄质前中期起促进积累作用,中后期起促进代谢作用;有机肥显著促进叶黄质、β-胡萝卜素积累。无机肥处理的LOX活性在烤烟发育前期高于有机肥处理,30天后,有机肥处理的LOX活性一直高于无机肥处理。有机肥调整了发育末期类胡萝卜素类物质组分比例构成和色素与相关酶类的关系。试验表明,有机肥条件下β-胡萝卜素、叶黄质含量较多是烤烟香气量较多的前提,烤烟发育后期LOX活性较高是有机肥促进烤烟品质的生理基础。

Pot experiment was conduction to study the effect of organic N on the carotenoids metabolism and LOX activity of growing flue-cured tobacco leaf. Results obtained are as follows： The content of Carotenoids has a high level during the growth of flue-cured tobacco leaf under the organic N condition. During the former and metaphase the content of neoxanthin under the organic N condition were more, but conditions were changed during the later anaphase. The content of lutein and β-carotene in condition of organic N were remarked more than theirs in the mineral N condition. During the former phase of flue-cured tobacco leaf the activity of LOX（mineral） were higher, but after 30 days the activity of LOX（organic） were remarkable higher than minerals. Proportion of the carotenoids and the relation of the pigment and relating enzyme had changed under the organic N. The experimentation indicated that β-carotene, neoxanthin were more were the one biochemistry reason of the aroma more under the organic N, and activity of LOX has a high level during the latter growth phase were the one physiology foundation of the better quality of flue-cured tobacco leaf under the organic N in Henan province.