摘要
目的建立枳椇子中黄酮类化合物的HPLC-ESI-MS分析方法,鉴定枳椇子中黄酮类化合物。方法色谱柱:Acquity UPLCTM BEH C18(50mm×2.1mmID,1.7μm);流动相:甲醇(A):0.1%甲酸水溶液(B),梯度洗脱;紫外检测波长290nm;采用正离子检测模式。结果枳椇子中的5个黄酮类化合物获得了良好的分离与检测,通过与对照品的保留时间、正离子质谱数据和文献数据初步鉴定出5个主要的黄酮化合物。结论该方法准确快速,适合枳椇子中黄酮类化合物的鉴定。
Objective To establish a HPLC - ESI - MS method for simultaneous analysis and determination of flavonoids in Semen Hoveniae. Methods The separation was performed on Acquity UPLCTM BEH C18 column （50 mm × 2. 1 mm, 1.7 μm） with a mobile phase of formic acid aqueous solution （B） and MeOH （A）. The flow rate was at 1.0 mL/min and detection wavelength was at 290 nm. Samples were analyzed in positiveion detection model. Results Five kinds of flavonoids in Semen Hoveniae were separated well and were tentatively identified by ESI - MS and by reference to litera- ture data. Conclusion The method is accurate and rapid, and can be used for the identification of flavonoids in Semen Hoveniae.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第4期341-343,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
