日本血吸虫不同抗原检测家兔感染及治疗前后IgG/IgM抗体动态

The dynamic observations on the development of IgG/IgM antibodies before and after treatment of rabbit infection with Schistosoma japonicumusing different kinds of schistosomal antigens

目的探索特异性抗体检测在日本血吸虫病诊断和疗效考核中的潜在应用价值。方法ELISA方法采用可溶性虫卵抗原(SEA)、成虫抗原(AWA)检测人工感染家兔治疗前后血清中特异性IgGI、gM抗体,评价特异性抗体检测的诊断和疗效考核价值。结果用SEA抗原检测IgM抗体,发现感染后7周不管治疗与否,抗体水平均快速下降。与SEA相比,感染后AWA特异性IgM抗体上升时间早,且治疗后5个月仍为阳性。SEA特异性IgG抗体水平最高,但治疗后5个月仍为阳性。结论采用SEA抗原检测IgG用于血吸虫病诊断效果较好;对早期感染的诊断可以考虑SEA抗原检测IgM抗体作辅助。AWA抗原检测IgM,对急、慢性血吸虫感染均有较好诊断效果。采用这两种抗原检测IgG和IgM,均无考核疗效意义。

To determine the diagnostic value as well as the evaluation value in therapy, the specific IgM and IgG antibodies of the infected and treated rabbits were detected using soluble egg antigen（SEA） and adult worm antigen（AWA） by ELISA. By using SEA to detect the IgM antibodies, the serum antibody level rapidly dropped 7 weeks after infection even without treatment, and those of IgM in sera detected by AWA rose early than IgG detected by SEA. After 5 month treatment, IgM detected by AWA and IgG detected by SEA were still positive. From these observations, it is evident that SEA is a better antigen to detect specific IgG in the diagnosis of schistosomiasis, AWA is a better one for early diagnosis. The use of AWA as antigen to detect IgM showed high diagnostic value both in acute and chronic schistosomiasis. However, detection of the IgM/ IgG in ELISA using SEA and AWA could not evaluate the therapeutic effect well.