中药材黄芪的高效液相色谱指纹图谱研究

HPLC Fingerprint Spectra of Radix Astragali of Different Sources

目的:对山西省浑源县不同购买批次的黄芪药材指纹图谱进行比较研究。方法:应用HPLC法,色谱柱:Thermo ODS(2.1mm×150mm,5μm);流动相:乙腈-水(3∶7);流速0.8mL/min;检测波长254nm;柱温:30℃;分析时间:60min;进样量:20μL。结果:山西省浑源县3种批号的黄芪药材有13个共有峰,其HPLC指纹图谱基本一致,但共有峰面积的比值有一定差异。结论:通过HPLC法建立了黄芪药材的指纹图谱分析方法,该方法稳定、可靠、重现性好,为快速鉴别区分不同来源的药材提供了科学的依据。

Objective：To study comparatively on HPLC fingerprint spectra of 3 batches of Radix Astragali from Hunyuan County, Shanxi Province. Method： HPLC was performed on Thermo ODS column （2.1 mm×150 mm, 5 μm）with acetonitrile-water （3：7）as mobile phase at flow rate of 0.8 mL/min. The column temperature was 30 ℃ and the detection wave- length was 254 nm.Analyzing time was 60 min and specimen handling quantity was 20 μL. Result：3 batches of Radix Astragali from Hunyuan County,Shanxi Province had 13 characteristic common peaks,but the ratios of common peaks area were different. Conclusion：Fingerprint spectra of Radix Astragali was constructed by HPLC. This method is stable,reliable, reproducible, and provides scientific basis for herbal discrimination.