实验猴结核病免疫学诊断抗原与诊断方法研究进展

Progress on Immunodiagnostis Antigens and Methods in Tuberculosis of Experimental Monkeys

结核分支杆菌H37Rv株的全基因组序列解码加速了对重组抗原的克隆和表达,LAM、38 ku抗原1、6 ku抗原、ESAT-6、CFP-10和Mtb8.4等特异性抗原的出现,弥补了经典抗原OT和PPD在实际应用中的不足,丰富了TST、LTT、ELISA和IFN-γ检测等诊断方法的内容,提高了检测的准确性。论文结合免疫学诊断抗原在各诊断方法中的应用,概括分析了各抗原的敏感性和特异性。鉴于检测经费、仪器设备和检测频率的限制,目前TST仍是实验猴场最实用的监测方法,而其他诊断方法在阳性猴的确诊和实验室研究中仍发挥着重要作用。

Since the entire genome sequence of Mycobacterium tuberculosis H37Rv was decoded, great progress has been made in cloning and expression of recombinant antigens. The LAM, 38 ku, 16 ku, ESAT- 6, CFP-10, MtbS. 4 and so on not only made up for the deficiencies of OT and PPD, but also enriched the immunodiagnostic methods such as TST,LTT, ELISA and IFN-γ assay, and improved the detection accuracy. The research progress on antigens and methods was summarized, meanwhile, the merits and disadvantages of different antigens and methods were depicted in this article. For the limit of funding, instruments and frequency, TST is still the most useful method,while the new methods play important roles in confirming the positive monkeys and lab research presently.