芥子气诱导人真皮成纤维细胞凋亡的研究

Study on apoptosis of human dermal fibroblasts induced by sulfur mustard

目的:研究芥子气诱导真皮成纤维细胞凋亡的作用,探讨其治疗银屑病的作用机制。方法:采用MTT法检测不同浓度芥子气对真皮成纤维细胞增殖的抑制作用,采用Annexin V-FITC法检测芥子气对真皮成纤维细胞凋亡指数的影响,以免疫组化法考察芥子气对肿瘤坏死因子相关凋亡诱导配体受体1(TRAILR1)表达的影响。结果:不同浓度的芥子气对真皮成纤维细胞的增殖均有抑制作用(P<0.01),并呈明显的剂量依赖性。真皮成纤维细胞的凋亡指数随着芥子气浓度的增加而增加。免疫组化考察结果表明,在芥子气的作用下,TRAILR1在人真皮成纤维细胞的胞膜和胞浆成强阳性表达。结论:芥子气可抑制真皮成纤维细胞的增殖,诱导其凋亡,该效应可能与芥子气促进TRAILR1的表达有关,从而可用于银屑病的治疗。

Objective： To study the inducing effect of sulfur mustard on apoptosis of human dermal fibroblasts and explore its mechanism of treating psoriasis. Methods： The inhibitive effect of sulfur mustard on proliferation of human dermal fibroblasts was detected by MTT method. Its effect on apoptotic index of human dermal fibroblasts was examined by Annexin VFITC method. The influence of sulfur mustard on the expression of tumor necrosis factor （TNF）-related apoptosis-inducing ligand receptor 1 （TRAILR1） in human dermal fibroblasts was examined by immunohistochemical method. Results： Different concentrations of sulfur mustard had significant and dose dependent inhibitive effect on the proliferation of human dermal fibroblasts （P〈0. 01）. The apoptotic index of human dermal fibroblasts increased as the concentration of sulfur mustard was elevated. The positive correlation between the concentration of sulfur mustard and expression of TRAILR1 protein in human dermal fibroblasts was observed. Conclusion： Sulfur mustard can inhibit the proliferation of human dermal fibroblasts and induce its apoptosis, which might be related to its enhancing effect on the expression of TRAILR1. This is a possible mechanism of sulfur mustard for treating psoriasis.