血浆肌红蛋白免疫比浊检测法中精密度和干扰实验的评价

Immunoturbidimetric detection of blood plasma myoglobin:evaluation of accuracy and interference test

目的对人血浆肌红蛋白(Mb)免疫比浊检测法进行评价,进而探讨该方法早期诊断急性冠状动脉综合征的临床价值。方法对肌红蛋白免疫比浊法的精密度和干扰实验进行评价。其中精密度、干扰评价按照美国临床实验室标准化委员会(NCCLS)评价方案进行。结果免疫比浊法的精密度良好,Mb高、中、低(410ng/ml、100ng/ml、40ng/ml)三个水平的批内变异、批间及总变异均小于5%。脂血、黄疸、溶血对实验结果基本无影响。结论免疫比浊法检测人血浆中的肌红蛋白准确、灵敏、可靠,可以很好地应用于急性冠状动脉综合征的早期诊断和溶栓疗效的监测。

Objective To evaluate the immunoturbidimetfic assay for the detection of blood plasma myoglobin and discuss its clinical utility for the early diagnosis of acute coronary syndrome. Methods Immunoturbidimetry was performed to detect blood plasma myoglobin. Accuracy and the result of interference test were evaluated according to NCCLS guideline. Results The accuracy of myoglobin immunoturbidimetric assay was good. The inter- batch and intra -batch variations and the total variations were all less than 5% in high, medium and low myoglobin levels （410 ng/ml,100 ng/ml and 40 ng/ml respectively）. Lipidemia, jaundice and hematolysis did not interfere with the result of myoglobin immunoturbidimetric assay. Conclusion Immunoturbidimetric detection of blood plasma myoglobin is accurate, sensitive and reliable. It can be used in the early diagnosis of acute coronary syndrome and monitoring of the efficacy of thrombolysis.