摘要
目的探讨肝细胞癌中Caveolin-1表达与上皮．间叶样表型转化（EMT）之间的关系。方法应用逆转录一聚合酶链反应（RT—PCR）、Western blot和免疫组织化学技术检测人肝癌细胞株和肝细胞癌组织标本中Caveolin-1和EMT指标mRNA和蛋白的表达。结果RT—PCR和Western blot结果显示，在HepG2、SMMC7721细胞株中，Caveolin-1mRNA表达量（0．03±0．01、26．63±0．49）和蛋白表达量（0．00±0．00、1．56±0．17）均与上皮细胞标志物E-cadherin（14．97±0．03、0．06±0．00）、（1．04±0．06、0．00±0．00）负相关，与间叶细胞标志物N—cadherin（2．03±0．07、9．54±0．05、0．68±0．09、0．95±0．05）、Vimentin（0．13±0．06、6．13±0．68、0．00±0．00、1．09±0．15）正相关。免疫组织化学结果显示，在82例肝细胞癌组织标本中，转移组癌组织中Caveolin-1表达明显低于癌旁组织（P〈0．05），且与上皮细胞标志物ZO-1、E—cadherin负相关，与间叶细胞标志物N－cadherin正相关。结论肝细胞癌中Caveolin-1表达升高与转移和EMT有关。
Objective To study the relationship between the expression of Caveolin-1 and epithelial-mesenchymal transition （EMT） in hepatocellular carcinoma （HCC）. Methods The protein and mRNA expression of Cavcolin-1 and markers of EMT in human HCC cell lines and tissue specimens of HCC was detected by using immunohistochemistry, Western blot, and real time PCR. Results In HepG2 and SMMC7721, Caveolin-1 mRNA （0.03 ± 0.01 and 26.63 ± 0.49 ） and protein （0.00 ± 0.00 and 1.56 ± 0.17 ） expression was negatively correlated with E-cadherin, but positively with N-cadherin and Vimentin. In tissue specimens of HCC, the Caveolin-1 expression in tumor tissues was significantly lower than that in adjacent tumor tissues, and correlate negatively with ZO-1 and E-cadherin, but positively with N-cadherin. Conclusion Overexpression of Caveolin-1 is correlated with metastasis and EMT in HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第8期970-972,共3页
Chinese Journal of Experimental Surgery
基金
福建省自然科学基金资助项目（2008J0083）
