三氧化二砷对肝癌细胞CD44v6、Ezrin基因表达的影响

The effects of arsenic trioxide on CD44v6 ,and ezrin gene expression in human hepatocellular carcinoma cells in vitro

目的观察肝癌HepG2细胞转移相关基因CD44v6、EzfinmRNA表达及三氧化二砷（As2O3）对其表达的影响。方法以逆转录-聚合酶链反应（RT—PCR）方法检测HepG2细胞中CIM4v6、Ezfin基因mRNA的表达及As203作用后其表达水平的变化。结果HepG2细胞CD44v6及EzfinmRNA均有表达。CIM4v6-mRNA表达水平为1．57±0．47；经0．25mg／L As2O3作用4、6、8d后其表达水平分别下降到0．95±0．25、0．90±0．24和0．63±0．19（P〈0．05），下降水平呈时间依赖；Ezfin．mRNA表达水平为0．70±0．18，加入0．25mg／L的As20，4、6、8d后，表达水平分别为0．70±0．21、0．75±0．22和0．71±0．20，各时间段Ezfin—mRNA的表达水平无明显变化（P〉0．05）。结论As2O3可显著下调HepG2细胞CD44v6-mRNA的表达，对Ezfin-mRNA的表达可能无直接调节作用。

Objective To investigate the expression of CD44v6,and Ezrin mRNA in human hepatocellular carcinoma cells （HepG2） ,and evaluate the effects of arsenic trioxide （As2O3 ） on the expression in HepG2 in vitro. Methods The expression levels of CD44v6 and Ezrin mRNA were examined by RT-PCR in HepG2 cells in vitro. Results The expression of CD44v6 and Ezrin mRNA was detected in HepG2 cells. The expression levels of CD44v6 and Ezrin mRNA were 1.57±0.47 and 0.70 ±0.18,respectively. A significant down-regulation of expression of CD44v6 mRNA was observed in HepG2 cells when 0.25 mg/L As2 O3 was given at the 4th,6th, and 8th day （0.95 ± 0.25,0.90 ± 0.24, and 0.63 ± 0.19 ,P 〈 0.05）. No change was found in the expression of Ezrin mRNA when HepG2 ceils were treated with As2O3 for 4,6, or 8 days （0.70 ± 0.21,0.75 ± 0.22, and 0.71 ± 0.20, P 〉 0.05 ）. Conclusion As2O3 can decrease the expression of CD44v6 mRNA, but it can not directly regulate the expression of Ezrin mRNA in HepG2 ceils in vitro.