载脂蛋白M在肝癌组织中的表达

Expressions of apolipoprotein M in hepatocellular carcinoma tissues

目的分析肝细胞肝癌患者血浆中载脂蛋白M（ApoM）的水平，观察ApoMmRNA水平在肝癌癌组织与癌旁组织中的表达。方法使用Dot—blot技术检测36例肝癌患者及64例正常人血浆中ApoM的水平，应用实时逆转录-聚合酶链反应（RT-PCR）检测ApoMmRNA在肝癌癌组织与癌旁组织中的水平，并采用Elivision TMplus免疫组织化学染色法对肝癌癌组织与癌旁组织ApoM组织学分析。结果肝癌患者血浆中ApoM的水平为（0．61±0．30）ODu·mm^-2，明显高于正常人（0．37±0．07）ODu·mm^-2，两者差异有统计学意义（P〈0．01）。肝癌组织ApoMmRNA表达明显低于肝癌癌旁组织水平（P〈0．05），免疫组织化学染色进一步证实了肝癌组织ApoM阳性显色低于癌旁癌组织。结论肝癌癌组织ApoMmRNA水平明显低于肝癌癌旁组织，而血浆中ApoM的水平明显高于正常人。

Objective To analyze plasma apolipoprotein M （ApoM） level and hepatic apoM mRNA level in the hepatocellular carcinoma （HCC） patients. Methods Plasma apoM levels were measured by the dot-blot analyses in 36 HCC patients, and 64 normal subjects as control. ApoM mRNA levels were detected in the HCC tissues and paracancerous normal liver tissues by RT-PCR, and further conformed by an immunohistochemistry staining method. Results The relative levels of plasma apoM in HCC patients were （0.61 ± 0. 30） ODu·mm^-2, which were significantly higher than those in the normal subjects [ （0.37 ±0.07） ODu·mm^-2 ] （P 〈0.01 ）. apoM mRNA levels in HCC tissues were significantly lower than those in the paracanemus normal tissues （P 〈 0.05 ）, which was further confirmed by the immunohistochemistry staining. ApoM positive staining in HCC tissues was significantly weaker than that in the cancerous normal tissues. Conclusion In HCC patients, apoM mRNA levels in tumor tissues were significantly lower more than those in the paracancerous normal tissues, and plasma apoM levels were significantly higher than those in normal subjects.