番茄抗青枯病种质资源遗传多样性的AFLP分析

Genetic diversity of tomato germplasm resources resistant to bacterial wilt (Ralstonia solanacearum) revealed by AFLP.

利用银染AFLP分子标记技术,对不同来源的18份番茄青枯病抗病材料和2份感病材料进行亲缘关系和遗传多样性分析.从64对引物中筛选出扩增效果稳定、多态性好的5对引物组合分别对20份材料的基因组DNA进行扩增,共扩增出清晰的条带201条,其中74条具有多态性,多态性位点百分率为36.8%.这一结果表明供试的抗青枯病番茄材料在DNA水平上的酶切位点分布存在一定的差异.应用NTSYS软件计算供试材料间的遗传相似系数,其变化范围为0.76～1.00.以遗传相似系数0.88为阈值进行UPGMA聚类分析,20份供试材料分成2个复合组和5个独立组.以上结果在DNA水平上为番茄抗青枯病育种的亲本选配提供了参考依据.

The relationship and genetic diversity of 20 tomato germplasm resources resistant or susceptible to bacterial wilt （Ralstonia solanacearum ） were analyzed by AFLP （amplified fragments length polymorphism）-silver staining approach. Five primer combinations selected from 64 primer pairs revealed a total number of 201 unambiguous bands, 74 of which were polymorphic with a polymorphism frequency of 36.8 %. This result showed moderate variations of some enzyme digestion sites among the used tomato germplasm resources. As analyzed by NTSYS software, the genetic similarity coefficient of 20 tomato resources ranged from 0.76 to 1.00. Based on the coefficient value of 0.88, these tomato germplasm resources were clustered into 2 multiple-member groups and 5 one-member groups by UPGMA （unweighted pair group method with arithmetic mean） analysis, which provided molecular reference for parent selection in breeding tomato for resistance to bacterial wilt.