刺楸组培快繁及试管苗保存培养基的筛选

Screening of culture medium for micropropagation and conservation in vitro of Kalopanax septemlobus Koidz.

以刺楸嫩叶柄为外植体,应用均匀设计法筛选最适合的培养基.结果表明,刺楸组织培养的不同阶段需要不同的培养基.最适的愈伤组织诱导培养基为C2D+6-BA(6-苄氨基腺嘌呤)0.50 mg·L-1+NAA(萘乙酸)1.00 mg·L-1;愈伤组织增殖培养基为C2D+6-BA0.50 mg·L-1+NAA 0.50～1.00mg·L-1;愈伤组织诱导再分化培养基为C2D+6-BA2.50 mg·L-1+KT(激动素)4.50 mg·L-1+NAA0.10 mg·L-1;生根培养基为1/2 MS+IBA(吲哚丁酸)0.10 mg·L-1+IAA(吲哚乙酸)0.10 mg·L-1;试管苗保存培养基为1/5 MS+ABA(脱落酸)2.50 mg·L-1.常温条件下,利用低营养促成休眠的方法在试管内保存刺楸种质可达44个月.

The tender leafstalk of Kalopanax septernlobus Koidz. was used as explant and its suitable medium compositions were screened through a uniform design method. The results show that tissue culture of Kalopanax septemlobus Koidz. required different kinds of culture medium in different phases. The most suitable culture mediums were as follows： C2D＋6-BA（6-henzyladenine） 0.50 mg. L^-1 ＋NAA （α-naphthaleneacetic acid） 1.00 mg. L^-1 for callus induction; C2 D＋ 6-BA 0.50 mg. L^- 1 ＋ NAA 0.50- 1.00 mg.L^-1 for callus multiplication; C2D＋ 6-BA 2.50 mg. L^- 1 ＋ KT（6-furfurylaminopurine） 4.50 mg.L^-1 ＋NAA 0.10 mg.L^-1 for callus redifferentiation; 1/2 MS＋IBA（indole-3 butyric acid） 0.10 mg.L^-1 ＋IAA（indole-3-aeetic acid） 0.10 mg.L^- 1 for rooting; 1/5 MS＋ABA（abscisic acid） 2.50 mg. L^-1 for conservation in vitro of shoots. These plant materials could be maintained for 44 months by the methods of promoting dormancy and low nutrients at normal temperature.