不同倍性蝴蝶兰杂交种子的离体培养

Aseptic Culture of the Hybrid Seeds of Different Ploidy Phalaenopsis

研究了影响不同倍性蝴蝶兰杂交种子离体培养的因素。结果表明,播种方式、培养基、光照时间、温度和杂交组合明显影响蝴蝶兰原球茎产量。播种方式、培养基和温度对蝴蝶兰原球茎百粒重影响显著。有机添加物对蝴蝶兰试管苗生长发育影响显著。适合不同倍性蝴蝶兰杂交种子的培养方案是采用稀释法将蝴蝶兰种子播种到1/2MS+6-BA0.2mg/L+NAA0.2mg/L+CM100ml/L培养基上,在27℃下培养;播种后15天内每天光照5h,15天后光照16h,光强为20.27～13.51μmol/(m2.s);60天后将小苗转移到1/2MS+NAA0.2mg/L+土豆汁30g/L+AC0.5g/L上壮苗,180天后炼苗移栽。

The factors influencing on the aseptic culture of Phalaenopsis hybrid seeds were studied. The results showed that sowing method, culture medium, light time, temperature and cross combination had obviously effects on the yield of protocorm body （PB）. Sowing method, culture medium and temperature had significant effects on 100-grain weight of PB. Organic additives had significant effects on the growth and development of PB. The suitable culture method for the hybrid seeds of different ploidy Phalaenopsis was as follow： seeds diluted with aseptic water were sown on 1/2 MS medium supplemented with 6-BA 0.2 mg/L,NAA 0.2 mg/L and CM 100 ml/L and cultured at 27 ℃ under light intensity of 20.27 - 13.51/zmoL/（ m^2 · s） and five hours per day for the first 15 days followed by sixteen hours per day for the next 45 days. Seedlings were then transferred to the medium of 1/2 MS ＋ NAA0.2 mg/L ＋ potato juice 30 g/L ＋ AC 0.5 g/L for plantlets development,after 180 days,the plantlets could be transplanted.