响应面法优化南强菌素发酵培养基

Optimization of Medium for Deacetylmycoepoxydiene Fermentation by Response-surface Methodology

从海洋真菌Phomopsis sp.A123分离得到的南强菌素(Deacetylmycoepoxydiene)是2003年新发现的具有抗肿瘤活性化合物.采用单因素筛选方法,筛选得到影响该化合物产生的4个因素,分别为葡萄糖、维生素B1、马铃薯和甘露醇.在此基础上,采用响应面法对4个因素最佳水平范围进行研究,利用Design-Expert软件进行2次回归分析,在培养基中葡萄糖、维生素B1、马铃薯和甘露醇的含量分别为87,0.005,170和14 g/L时,南强菌素的产量从35 mg/L提高到200 mg/L.

Deacetylmycoepoxydiene（DAM） ,isolated from the marine fungus Phornopsis sp. A123,is a novel antitumor compound. It was first isolated in 2003. In the present work,after single-factor optimization,four key factors with strong impact on the production of DAM,namely,glucose,potato,vitamin B1 and mannitol were selected. The centre composition design of response surface method was applied to optimize the best concentration of the four factors. The optimization levels of the various ingredients were：glucose 87 g/L,potato 170 g/L,vitamin B1 5 mg/L and mannitol 14 g/L,and the predicted yield of DAM in liquid medium was 220 mg/L. After applying the quadratic regression model equation, the actual yield of DAM reached 200 mg/L, this was 6 times that of the initial medium.