葡萄糖氧化酶催化反应动力学特征及其抑制剂

Kinetic Characteristics of Catalytic Reaction ofGlucose Oxidase and Effect of Inhibitor on Rate

研究了葡萄糖氧化酶—过氧化物酶—４－氨基氨替比林－２，４－二氯苯酚酶偶联反应的动力学性质及一部分金属离子对反应的影响。Ａｇ＋、Ｈｇ２＋离子是该酶催化反应的较强抑制剂，最小抑制浓度分别为５μｇ·Ｌ－１及４ｍｇ·Ｌ－１，Ｉ５０分别为２０μｇ·Ｌ－１及７７ｍｇ·Ｌ－１。

The kinetic characteristics of the catalytic reaction system of glucose oxidase-peroxidase-4-AAP-2,4-dichlorophend and effect of metal ion on reaction rate were studiedIt was found that Ag+ and Hg2+ are strong inhibitors to this reactionThe minimum inhibition concentraction is Ag+(5 μg·L-1),Hg2+(4 mg·L-1) and I50(50% inhibition) is Ag+(20 μg·L-1),Hg2+(77 mg·L-1)