毛细管电泳法测定灵芝中核苷类成分的含量

Determination of nucleosines in Ganoderma by CE.

目的建立毛细管电泳法测定灵芝中核苷类成分含量的方法。方法采用Agilent毛细管电泳仪，熔融石英毛细管56cm×75μm，有效长度50cm,自动压力（30mPa）进样2s，检测波长254nm，分离电压27kV，运行缓冲液为0．05mol／L，硼砂-甲醇（82：18）。结果灵芝中主要成分腺苷、鸟苷、尿苷、肌苷分别在2．33～116．6mg／L，2．08—104．2mg／L，2．06～103．0mg／L，1．08～43．2mg／L范围内有良好的线性关系，相关系数r分别为0．9998、0．9997、0．9996、0．9998。加样回收率分别为97．6％、101．2％、102．7％、101．4％。RSD分别为2．1％、1．6％、2．8％、2．3％。结论此法简便、快速，适用于灵芝中核苷类成分含量的同时测定。

Objective To establish CE method for making a assay of nucleosines in Ganoderma. Methods Agilent apparatus equipped with a UV detector and a untreated fused- quartz capillary（ 56cm × 75μm,50cm effective length）were used. Pressure injection was 30mpa for 2s, and the wavelength of detector was 254nm. 27kV was applied for separation. The running buffer was 0.05mol/L^-1 Sodium borate - Methanol （82：18）. Results The Linear ranges of adenosion,guanosion,uridine,inosion lay in the concentration range of 2.33 - 116.6mg/L,2.08 104.2mg/L,2.06 - 103.0mg/L, 1.08 - 43.2mg/L, respectively, r = 0. 9998,0. 9997,0. 9996,0. 9998. The average recoveries were 97.6% ,101.2%, 102.7% , 101.4%, RSD were 2.1% , 1.6% ,2.8% ,2.3% , respectively. Conclusion The method is simple, rapid. It is suitable for the assay of nucleosions in Ganoderma.