微生物检定法测定莫能菌素的含量

Determination of monensin by microbial assay

试验建立一种新的抗生素微生物检定法测莫能菌素预混剂含量的方法。以无水甲醇为溶剂，超声浸提30min，以枯草芽孢杆菌为试验菌，用pH值6．0灭菌缓冲盐溶液为稀释液，按抗生素微生物检定法检测，莫能菌素浓度在2～10U／ml范围内抑菌圈直径与浓度的对数之间线性关系良好。结果表明：其相关系数为0．9983（n=8），准确度为90．0％～110．0％，分析精密度为RSD2．8％～2．9％，人员精密度为RSD为2．9％。此方法与国标法相比，甲醇用量少，降低环境污染，快速、准确、可靠。

To establish a new microbial assays of monensin premix. Method： anhydrous methanol as the solvent, ultrasonic extraction for 30 minutes and Bacillus subtilis as the test bacteria, pH6.0 sterile saline buffer as diluent, according to antibiotic microbial assay detection, the linear relationship between inhibition zone diameter and logarithm of the concentration in 2-10 U/ml of monensin is good. results： Correlation coefficient 0.998 3 （n=8）, Accuracy： 90.0%-110.0%, analytical precision： RSD 2.8%-2.9%, personal precision： RSD 2.9%. Conclusion： compared with national standard method, the method uses less methanol, and reduce environmental pollution, It＇s a rapid, accurate and reliable method.