灰阶谐波超声造影评价兔肾动脉狭窄的实验研究

Experimental study of real-time contrast-enhanced ultrasound harmonic gray scale imaging in the evaluation of renal artery stenosis in rabbits

目的评价实时灰阶谐波超声造影在诊断兔肾动脉狭窄模型中肾动脉狭窄的价值。方法手术建立10只兔肾动脉狭窄的模型，其中6只右肾动脉，4只左肾动脉。兔造模前接受常规超声检查；造模后，接受常规超声检查及灰阶谐波超声造影检查。其结果与数字减影血管造影检查对照。结果谐波超声造影检查肾动脉狭窄24％～69％的4只，70％～89％的5只，1只兔子因麻醉过深而死亡；数字减影血管造影证实。肾动脉狭窄31％～64％4只，71％～93％的5只；两者有很好的相关性（r=0．97）。结论实时灰阶谐波超声造影显示的管腔结构较二维及彩色多普勒超声等检查更接近于实际管腔，有望成为判断肾动脉狭窄程度的新方法。

Objective To explore the value of contrast-enhanced real-time harmonic gray scale imaging in evaluation of renal artery stenosis in rabbits. Methods Renal artery stenosis models were made in 10 rabbits by operation, including 6 right renal artery stenosis and 4 left renal artery stenosis. All rabbits were examined by ultrasound before operation, then examined by conventional ultrasound and contrast-enhanced real-time harmonic gray scale imaging after the operation. The results were compared with that of DSA （digital subtraction arteriography ）. Results By contrast-enhanced real-time harmonic gray scale imaging examination, there were 4 rabbits with stenosis grade of 24% -69% ,5 rabbits with stenosis grade of 70% - 89% , and one rabbit died of deep anaesthesia. While by DSA examination, there were 4 rabbits with stenosis grade of 31% -64%, 5 rabbits with stenosis grade of 71% - 93%. There was good correlation between the two methods （ r = 0.97 ）. Conclusion Contrast-enhanced real-time harmonic gray scale imaging can be used to observe renal artery stenosis in rabbits, it might be a new method for diagnosis of renal artery stenosis.