本草消渴丹对实验性糖尿病大鼠糖代谢酶活性的影响

Effect of Bencao Xiaoke Pill on Carbohydrate Metabolism Enzyme Activity in Type 2 Diabetic Rats

目的探讨中药复方—本草消渴丹对2型糖尿病大鼠糖代谢的关键酶己糖激酶、苹果酸脱氢酶活性的影响,为临床用药提供科学依据。方法选择高脂、高糖饲料加小剂量链脲佐菌素(STZ,25mg/kg,腹腔注射)的方法建立大鼠2型糖尿病模型,分别以本草消渴丹和西药二甲双胍进行干预,每周末断尾取血检测空腹血糖值。治疗4周后检测大鼠空腹血糖值、肌肉己糖激酶(HK)和肝脏苹果酸脱氢酶活性(MDH)。结果本草消渴丹治疗可以显著降低糖尿病大鼠血糖,其肌肉组织HK、肝脏组织MDH活性与模型组对比有显著性差异。结论中药复方—本草消渴丹降低血糖的作用机制之一可能是直接或间接提高了葡萄糖氧化分解的酶活性;本草消渴丹对对抗糖尿病体重降低有一定作用。

Objective To study the effect of Chinese Herbal Compound--Bencao Xiaoke pill on the activity of key enzymes - hexokinase and malate dehydrogenase in the carbohydrate metabolism of type 2 diabetic rats and provide scientific basis for clinical application. Methods Type 2 diabetic rat model was built by small dose injection of streptozotocin （STZ） （25 mg/kg, intraperito- neal injection ） and intake of high lipid die. After the model was established, the rats with diabetes were treated with Compound--Bencao Xiaoke pill and mefformin. Blood glucose was detected every weekend by cutting rats＇ tails. After four weeks treatment the blood glucose, activities of muscle hexokinase （ HK ） and liver malate dehydrogenase （ MDH ） were tested. Results The blood glucose was significantly decreased as compared with model groups, and there had significant differences in the activities of HK of the muscle tissue and MDH of the liver. Conclusion One of the hypoglycemic mechanism for materia medica Xiaoke pills is to increese directly or indirectly the activity of carbohydrate metabolic enzymes. The Compound--Bencao xiaoke pill has certain effect on the weight loss of diabetic body.