苏州地区急性呼吸道感染住院患儿病毒病原学分析

Viral pathogens of acute respiratory infection in hospitalized children from Suzhou

目的了解苏州地区急性呼吸道感染（ARI）住院患儿的病毒病原学情况。方法采集1668例7岁以下ARI住院患儿鼻咽分泌物，直接免疫荧光法检测呼吸道合胞病毒（RSV），流感病毒A，B型（IV A，B），副流感病毒1，2，3型（PIV1，2，3）及腺病毒A（ADA）等7种常见呼吸道病毒，用RT-PCR法检测人类偏肺病毒（hMPV）N基因。结果①1 668例患儿中，病毒检测阳性597例（35.8%）。单项病毒感染561例（33.6%），其中7种常见呼吸道病毒检出率为23.1%，以RSV为主（17.6%），好发于冬春季，主要见于1岁以下婴幼儿；②hMPV阳性率为10.6%，3-5月份为检出高峰期。③36例混合感染中，22例为RSV合并hMPV感染，主要见于1岁以下婴幼儿。结论RSV是该地区ARI的主要病毒病原，RSV感染好发于冬春季，多见于1岁以下婴幼儿，hMPV是3-5月份ARI患儿的主要病毒病原，混合感染多见于1岁以下婴幼儿。

Objective To investigate the viral pathogens of acute respiratory infection （ARI） in hospitalized children from Suzhou of China. Methods The nasopharyngeal aspirate samples were obtained from 1 668 hospitalized children with ARI. Common respiratory viruses, including respiratory syncytial virus （RSV） , influenza virus A and B, parainflnenza viruses 1, 2 and 3 and adenovirus, were detected using direct immunofluorescence. Human metapneumovirus （ hMPV ） gene fragments were detected by RT-PCR. Results Viral agents were identified in 597 cases （35.8%）. RSV was the most frequent （ 17.6% ）. RSV infection is more common in children less than 1 year old. A highest detection rate of RSV was found during winter and spring, hMPV was detected in 10.6% of the cases, with a peak detection rate between March and May. Single viral infection was found in 561 cases （33.6%） and mixed viral infection in 36 cases （ including 27 cases at age of less than 1 year）. RSV and hMPV co-infection was common （ n= 22）. Conclusions RSV is common pathogen of ARI in children from Suzhou. RSV viral activity peaks during winter and spring. The children at age of less than 1 year are susceptible to RSV. hMPV is also an important pathogen of ARI, with a peak detection rate between March and May. Mixed viral infection is common in children less than 1 year old.