放疗对宫颈鳞癌细胞凋亡及FHIT基因表达的影响

Effect of radiotherapy on cell apoptosis and FHIT gene expression of cervical squamous carcinoma cells

目的:探讨放疗对宫颈癌FHIT蛋白表达、细胞凋亡的影响及FHIT蛋白与细胞凋亡的关系。方法:采用免疫组织化学和TUNNEL标记法检测50例ⅡB-ⅢB期宫颈癌患者在放疗前、放疗中(Dt10、30Gy)和放疗结束后,宫颈癌组织中FHIT蛋白表达水平的改变与细胞凋亡情况。结果:50例患者在放疗Dt10、30Gy和放疗结束时FHIT蛋白表达的阳性率分别为56%、68%和84%,与放疗前的36%相比,表达率明显升高,差异有统计学意义(P<0.05);细胞凋亡阳性率分别为52%、64%和78%,与放疗前的28%相比,亦明显升高(P<0.05);随着放疗进展,细胞凋亡阳性率与FHIT蛋白表达的阳性率呈正相关(P<0.05)。结论:放疗能增强FHIT蛋白表达,同时诱导细胞凋亡;FHIT蛋白在放射线诱导的细胞凋亡过程中可能起调控作用。

Objective：To explore the effect of radiotherapy on the FHIT protein expression and cell apoptosis of cervical squamous carcinoma and discuss the relationship between FHIT protein expression and cell apoptosis. Methods ： Expression of FHIT protein was measured by immunohistochemical method and cell apoptosis was detected by TdT-mediated dUTP terminal nick end labeling （TUNEL） staining in 50 cases of squamous cell cervical carcinoma at ⅡB-ⅢB stages before, during （Dt 10 Gy and Dt 30 Gy）, and after radiotherapy. Results：Of the 50 patients, the positive rates of the expression of FHIT protein was 56% at Dt 10 Gy, 68% at Dt 30 Gy, and 84% after radiotherapy, which were significantly increased compared with that before radiotherapy （36%, P 〈0.05）. The positive rates of cell apoptosis was 52% at Dt 10 Gy, 64% at Dt 30 Gy and 78% after radiotherapy, which were significantly elevated compared with that before radiotherapy （28% , P〈0.05）. In the process of radiotherapy, cell apoptosis was positively related to the expression of FHIT protein （P〈0.05）. Conclusion： Radiotherapy reinforces the expression of FHIT protein and induces apoptosis cocurrently. FHIT protein has regulatory effects in cell apoptosis induced by radiotherapy.