CGRP肽能神经在皮肤组织中的分布及其意义

The distribution of calcitonin gene-related peptidergic nerve in skin tissue and its significance

目的:了解降钙素基因相关肽(calcitonin gene-related peptide,CGRP)在皮肤组织中的分布、超微定位,以探讨其在皮肤中功能影响。方法:采用ABC-GND免疫组化染色、免疫透射电镜方法观察CGRP在正常皮肤分布和超微结构情况。结果:CGRP分布在表皮下、毛囊、皮脂腺周围、真皮、皮下以及小血管壁周围,其中以小动脉壁阳性纤维密度大。可见粗、细两种纤维,前者位于真皮深层及皮下,后者位于表皮下、毛囊皮脂腺周围。四肢较胸背皮肤CGRP分布相对多而明显。结论:CGRP可能作为神经调节或神经递质参与皮肤各种生理功能调节。尤其在血管周围分布,可能作为CGRP调节皮肤的机制研究和可能的药物治疗的研究基础。

Objective： To study the distribution of calcitonin gene-related peptide （CGRP） nerve fibers in SD rat skin and explore its effects on skin function. Methods： Immunohistochemistry ABC-GND method and immuno-transmission electron microscope were used to explore the distribution of CGRP nerve fibers in SD rat skin tissue and its ultrastructural features. Results： CGRP positive nerve fibers located in epidermis, folliculus, glandulae sebaceae, dermis, cutaneous muscle and small blood vessels, with the highest density in small vessel walls. The CGRP nerve fiber was richer in limbs＇ skin than that in somatic skin. There were two kinds of CGRP nerve fiber in skin, big fibers, located in the deep layer of dema and epidermis, and fine fibers, in the surrounding of folliculus and glandulae sebaceae. Conclusions： As a kind of neuroregulator, CGRP deal with the physiological functions of skin tissue, which may provide clue for drug treatment of skin diseases.