交感神经对小鼠小肠T淋巴细胞亚型及其增殖的影响

The effect of sympathetic nerve on the intestinal subset and the proliferation of T lymphocyte in mice

采用化学阻断剂6-羟多巴胺(6-OHDA)损毁交感神经,研究了交感神经对小鼠小肠局部细胞免疫作用的内在机理。将30只雄性昆明小鼠随机分为2组,一组连续5d腹腔注射6-OHDA,另一组用作对照。采用免疫组织化学染色和细胞培养方法检测两组动物小肠CD4+、CD8+T淋巴细胞数量及肠系膜淋巴结T淋巴细胞体外增殖能力。结果表明,试验组的CD4+T细胞数量比对照组少6.5%～51.5%,十二指肠、空肠段差异极显著(P<0.01);CD8+T细胞数量比对照组增加5.6%～22.7%,十二指肠段差异显著(P<0.01);CD4+/CD8+T淋巴细胞的比值总体呈下降趋势。肠系膜淋巴结T淋巴细胞转化率降低,在ConA浓度为3μg/ml时比对照组降低了9.2%,差异显著(P<0.05)。以上结果提示:交感神经通过调节小鼠小肠T淋巴细胞增殖以及亚型CD4+、CD8+T淋巴细胞的数量,影响肠黏膜细胞免疫的调控。

The effect of sympathetic nerve on the mucosal immunity of small intestine of mice was studied by sympathectomy. A total of 30 male mice were randomly divided into two groups, one group as control and one group subjected to intraperitoneal injection of 6-OHDA for 5 days. The number of CD4^＋ and CD8^＋ T lymphocytes of intestine and the T lymphocyte proliferation of mescnteric lymph node were investigated by immunohistochemistry and cell culture methods. The results showed that the number of CD^＋ T cell in the experimental group decreased by 6.5 % - 51,5 % compared to control, with the significant difference appeared in duodenum and jejunum （ P 〈 0.05 ） , whereas that of CD8^＋ T cell increased by 5.6% - 22.7 % with the significant difference in duodenum （ P 〈 0.05 ）. Accordingly, the ratio of CD4^＋ to CD8 ^＋ tends to decrease in sympathectomy mice, T lymphocyte conversion ratio of mesenteric lymph node was 9.2% lower than control mice in response to ConA at 3 μg/ml （ P 〈 0.05 ）. These data indicate that sympathetic nerve can affect the cell immunity of small intestinal mucosa by regulating the proliferation of T lymphocyte and the relative number of CD4^＋ and CD8^＋ T cells.