高糖条件下Tensin在人肾脏系膜细胞上的表达

Tensin expression in mesangial cells of human kidney

目的:探讨在高糖刺激的条件下,Tensin在人类肾脏系膜细胞上的表达及变化。方法:体外培养系膜细胞,用含不同浓度的葡萄糖(5mmol/L,30mmol/L)刺激细胞48h、72h和5d。用免疫荧光法观察tensin在人类肾脏系膜细胞上表达及变化,ELISA法检测上清液中纤连蛋白含量。结果:高糖刺激下系膜细胞tensin的表达随着培养时间的延长逐渐增多,且纤连蛋白的分泌也随培养时间的延长而增加。结论:高糖刺激下tensin在人类肾脏系膜细胞的细胞膜上表达增加,在细胞外基质增多的发生机制中起重要作用。

AIM： To investigate the expression changes of tensin in human kidney mesangial cells with stimulating of high concentrations of glucose （HG）. METHODS： Human mesangial cells were cultured and stimulated with different concentrations（5 mmol/L, 30 mmol/L）of glucose for 48 h, 72 h and 5 d. The expression changes of tensin was detected by immunofluorescence technique, and fibronectin of supernatant were detected by ELISA. RESULTS： The expression of tensin in mesangial cells was more increased significantly with stimulating of high concentrations of glucose with different culturing time, and fibronectin was secreted into the supernatant and the concentrations were increased with culturing time. CONCLUSION： The expression of tensin is increased in mesangial cells with stimulating of high concentrations of glucose, and tensin plays an important role in pathogenesis of accumulation of extracellular matrix.