Graves病患者杀伤细胞免疫球蛋白样受体基因型分析

Genotype analysis of killer cell immunoglobulin-like receptors in Graves' disease patients

目的:探讨杀伤细胞免疫球蛋白样受体(KIR)基因型在Graves病(GD)患者中的分布规律。方法:采用序列特异性引物聚合酶链反应(PCR-SSP)的方法,分析96例Graves病患者和96例正常对照人群的KIR基因型。结果:GD患者中2DS2-,2DL2-,2DL3+,2DL1+,3DL1+,3DS1-,2DL5-,2DS3-,2DS5-,2DS1-,2DS4-基因型频率高于对照组(6.25%vs0,P<0.05);对照组中,基因频率最高的基因型为2DS2-,2DL2-,2DL3+,2DL1+,3DL1+,3DS1-,2DL5-,2DS3-,2DS5-,2DS1-,2DS4+,该基因型的基因型频率较GD患者组高,且有显著性差异(28.13%vs10.42%,P<0.01);GD患者组不携带活化性KIR基因的基因型频率较对照组明显升高(10.42%vs0%,P=0.001)。结论:KIR基因型在GD患者与正常人群分布的差异可能与GD的发病有关。

AIM： Killer cell immunoglobulin-like receptor （KIR） can modulate the activity of NK and T lymphocyte. To investigate whether the KIR genotype possessing a susceptibility to Graves disease（GD）. METHODS： Using PCR-SSP to detect KIR genotype in 96 GD patients and 96 randomly selected healthy controls. RESULTS： The genotype frequency of 2DS2^-, 2DL2^-, 2DL3^＋ , 2DL1^＋ , 3DL1^- , 3DS1^- , 2DL5^-, 2DS3^-, 2DS5^-, 2DS1^-, 2DS4^- was significantly higher in the patient group compared to that of the control group （6. 25% vs 0%, P 〈 0. 0.5）. Genotype of 2DS2^-, 2DL2^-, 2DL3^- , 2DL1^＋, 3DL1^＋ , 3DS1^-, 2DL5^-, 2DS3^-, 2DS5^-, 2DS1^-, 2DS4^＋ is the most prevalent in the controls （28. 13% vs 10.42%, P 〈 0.01）. Genotypes without activating KIR genes have higher frequency in patient group. CONCLUSION： The difference of KIR genotypes between GD patients and healthy controls may explain the pathogenesis of GD.