溶血磷脂酸对宫颈癌细胞增殖、黏附、迁移和凋亡的影响

Influce of lysophosphatidic acid on proliferation,adhesion,migration and apoptosis of cervical cancer HeLa cells

目的:研究溶血磷脂酸(LPA)对宫颈癌HeLa细胞增殖、黏附、迁移和凋亡的影响及其作用机制。方法:LPA作用于细胞后,细胞计数法观察细胞的增殖;MTT法测定细胞对顺铂的敏感性、流式细胞仪检测顺铂诱导的凋亡;tran-swell小室检测细胞迁移能力变化;黏附实验测定细胞黏附力;同时,观察抑制剂Y27632、LY294002、PD98059对LPA功能的影响。结果:LPA以时间和剂量依赖方式促进细胞增殖,MEK1抑制剂显著抑制LPA的促增殖作用;LPA以剂量依赖方式显著促进HeLa细胞的迁移、和对细胞外基质的黏附,Rho激酶抑制剂显著抑制LPA诱导的迁移和黏附;LPA保护顺铂诱导的细胞凋亡,PI3K抑制剂可显著阻止LPA此作用。结论:LPA可以通过多种信号传导通路途径促进宫颈癌细胞的增殖、迁移和黏附,并抑制细胞凋亡。

AIM： To observe the effect of lysophosphatidic acid on cervical cancer cell line HeLa cells proliferation, adhesion, migration and apoptosis and study related signaling pathway. METHODS： After HeLa cells affected by LPA, the level of proliferation was drawing grouth curve ater cell couting; the sensitivity to cisplatin was evaluated by methyl thiazolyl tetrazolium （MTT） assay and flow cytometry was used to examine cell apoptosis; the potentiality of migration was evaluated by transwell chamber, then to observe the effect of Y27632, LY294002 and PD08059 on biological function of LPA. RESULTS： LPA could promote HeLa cell proliferate on time-dependent and dose-dependent relationship. MEK1 inhibitor could inhibite LPA-induced cell proliferation; LPA promoted the adhesion and migrationof HeLa cells in dose-dependence; Rho inhibitor could inhibit the LPA-induced adhesion and migration; LPA could reduce the chemotherapy sensibility of cervical cancer to DDP in dose-dependence, then PI3K inhibitor could resist this effect. CONCLUSION： LPA could promote HeLa cells＇proliferation, adhesion, migration and anti-apoptotosis through multiple signaling pathways.