细胞角蛋白20的表达与乳腺癌进展、转移及预后的相关性

Association of CK20 expression with the progression,metastasis and prognosis of breast cancer

目的:探讨细胞角蛋白20(CK20)在乳腺癌组织中的表达及其与乳腺癌进展、转移和预后的相关性。方法:选取乳腺癌患者86例,以20例乳腺良性肿瘤组织作为对照,应用免疫组织化学染色方法检测CK20的表达,并分析其与乳腺癌临床病理特征及预后的关系。结果:CK20在乳腺癌组织中的阳性表达率为80.23%(69/86),明显高于在乳腺良性肿瘤中的表达[20.00%(4/20),P<0.01]。CK20的表达与乳腺癌组织学分级(P<0.05)和病理类型(P<0.01)有关,与TNM分期(r=0.86,P<0.05)、淋巴结转移(r=0.73,P<0.05)和HER-2(r=0.69,P<0.05)呈正相关,与ER(r=-0.58,P<0.05)呈负相关。CK20阳性表达组和CK20阴性表达组的5年生存率分别为38.46%(25/65)和66.67%(10/15),差异有统计学意义(P<0.01)。结论:CK20与乳腺癌的进展、转移有关,作为新的乳腺癌标记物,有一定的临床实际应用价值。

AIM： To analyze the expression of CK20 in human breast cancer, and to evaluate the association between its expression and tumor＇s progression and prognosis. METHODS： 86 cases with breast cancer, 20 cases of benign tumor tissues were examined for the expression of CK20 by immunohistochemical staining. RESULTS： The positive rate of CK20 expression in breast cancer was 80.23% （69/86）, which was significantly higher than that in benign tumor tissues of breast [ 20.00% （4/20）, P 〈 0.01 ]. CK20 expression was associated with the histologic grades （ P 〈 0.05） and the pathological types （ P 〈 0.01）. The expression of CK20 was observed to correlate positively with TNM stages （ r = 0.86, P 〈 0.05）, lymph node status （ r = 0.73, P〈0.05） and HER-2 status （r=0.69, P〈0.05）, and correlate negatively with ER status （ r = - 0.58, P 〈 0.05）. Moreover, Kaplan Meier curves of overall survival analysis showed a significant difference between CK20 positive groups and negative group （ P 〈 0.01 ）. CONCLUSION： The results suggest that the expression of CK20 may be associated with the progression and prognosis of breast cancer.