参芎注射液对腹膜纤维化的抑制作用

The inhibitory effects of shenxiong injection on peritoneal fibrosis

目的探讨参芎注射液改善腹膜纤维化的作用机制。方法将32只SD雄性大鼠随机分为4组：正常对照组（A组）、模型组（B组）、低剂量组（C组，参芎注射液15ml／kg）和高剂量组（D组，参芎注射液30ml／kg），每组8只。于实验第28天处死动物取腹膜组织待检。留取腹膜组织做HE及Masson染色，RT-PCR间接免疫荧光法检测细胞转化生长因子β1（TGF-β1）的表达。结果与A组相比，B组的壁层腹膜厚度明显增加；与B组相比，C组和D组的腹膜厚度明显减轻。B组腹膜组织TGF-β1表达明显上调，C组和D组腹膜组织TGF-β1表达明显减低。结论参芎注射液可通过抑制腹膜纤维化大鼠腹膜组织的TGF-β1表达而发挥显著的抑制纤维化的作用。

Objective To investigate the action mechanism of shenxiong injection in treating peritoneal fibrosis. Methods The model of peritoneal fibrosis was induced by high glucose dialysate （4. 25 %, Baster） and lipopolysaccharide （LPS）. The rats were randomly divided into 4 groups： control group, model group, low dose shenxiong injection-treated group and high dose shenxiong injection-treated group The shenxiong injection-treated groups received daily intraperitoneal injection of 15 and 30 ml/kg shenxiong injection respectively. All rats were sacrificed after 4 weeks. The abdominal membrane was stained with HE and Masson methods. The expression of TGF-β1 in peritoneal membrane was detected by using immuno-fluorescent and RT-PCR methods. Results The peritoneal membrane in model group was significantly thickened. As compared with model group, the peritoneal membrane in shenxiong injection-treated groups was thinned markedly. The expression of TGF-β1 was obviously up-regulated in model group as compared with model group, and that in low dose and high dose shenxiong injection-treated groups was notable decreased. Conclusions shenxiong injection plays a significant role in anti-fibrosis by inhibiting the expression of peritoneal TGF-β1.