摘要
目的:通过对磷脂水解产物溶血磷脂(LPC)的测定,对比空白脂质体与含药脂质体中溶血磷脂含量变化,系统研究了羟喜树碱脂质体中药物对脂质体稳定性的影响。方法:通过高温加速试验的方法,对HCPT脂质体中LPC含量的变化进行比较性研究,并采用HPLC-ELSD法对其含量进行准确测定。结果:经加速试验后发现,含药脂质体中LPC的百分含量较空白脂质体增加10%,两者存在显著性差异(P<0.05)。结论:HCPT脂质体中药物对磷脂水解过程有明显的催化作用,建议在此类药物的研发中应高度关注。
Objective： Lysophosphatidylcholine （ LPC）, a hydrolysis product of phospholipids （ PL）, regulates a broad range of unfavourable cell processes. In hydroxycamptothecin （HCPT）-containing liposomes, the LPC concentrations were test to find out whether HCPT can impact the stability of this liposome. Methods： PL-hydrolysis in accelerated test was monitored by HPLC-ELSD with LPC quantified. The fluorescence properties of HCPT liposome and control liposome were also explored. Results. LPC in HPCT-containing liposomes increased 10% than that in control liposomes. Conclusion： HCPT has a catalytic effect on PL hydrolysis. The increase of lysophosphatidylcholine should be concerned in the storage of HCPT liposome.
出处
《药学与临床研究》
2009年第4期306-308,共3页
Pharmaceutical and Clinical Research
关键词
溶血磷脂
磷脂
水解
羟喜树碱
脂质体
Lyso-phosphatidylcholine
Phospholipids
Hydrolysis
Hydroxycamptothecin
Liposome
