三七总皂苷对佐剂性关节炎大鼠的抗炎及免疫调节作用

Anti-inflammatory and Immunomodulation Effects of Panax Notoginseng Saponins in Rats with Adjuvant Arthritis

目的:研究三七总皂苷(panax notoginseng saponins,PNS)对佐剂性关节炎(rheumatoidarthritis,AA)大鼠的抗炎及免疫调节作用。方法:大鼠右后足跖皮内注射弗氏完全佐剂诱发大鼠AA模型,进行多发性关节炎评分,放射免疫法检测大鼠血清肿瘤坏死因子(tumor necrosis factor,TNF)、白细胞介素-1β(interleukin-1β,IL-1β)含量,同时对脂多糖(lipopolysaccharide,LPS)诱导的AA大鼠腹腔巨噬细胞(peritoneal macrophages,PM)产生的TNF、IL-1β进行检测。结果:大鼠免疫后2周,PNS腹腔注射给药2周,可降低AA大鼠多发性关节炎指数(P<0.05),抑制血清TNF(P<0.01)、IL-1β(P<0.05)产生;PNS体外分别给药0.8,0.4,0.2 mg/ml,作用24 h,可不同程度抑制LPS诱导的AA大鼠PM产生TNF(P<0.05,P<0.01)和及IL-1β(P<0.05)。结论:PNS可能通过抗炎及抑制血清、PM释放炎性细胞因子TNF、IL-1β对AA大鼠发挥治疗作用。

Objective：To investigate the anti-inflammatory and immunomodulation effects of panax notoginseng saponins（PNS） in rats with adjuvant arthritis （AA）. Methods： Complete Freund＇s adjuvant was used to induce AA in rats. Polyarthritis index were scored and levels of tumour necrosis factor-α （TNF-α） and interleukin-1β（IL-1β）from serum were determined by radio immunoassay. Meanwhile, levels of TNF-α and IL-1β produced by I.PS induced PM of AA rats were also determined by radio immunoassay. Results： PNS were given by intraperitoneal injection for 2 weeks from the 15th day after immunization . The polyarthritic symptoms of AA rats were improved significantly compared with those of AA model group（P〈 0.05）. PNS significantly reduced the enhanced productions of TNF-α（P〈0.01） and IL-1β（P〈0.05）from serum in AA rats. Meanwhile, PNS significantly reduced the enhanced productions of TNF-α（P〈0.05, or P〈0.01） and IL-1β（P〈0.05）produced by LPS-indueed PM of AA rats at different concentration （0. 8, 0.4 and 0.2 mg/ml, respedively）. Conclusion：PNS has therapeutic effect on AA in rats, which may be related to anti-inflammatory and immunomodulation effects of inhibiting productions of proinflammatory cytokines of TNF-α and IL-1β from serum and LPS-induced PM.