生脉散对LPS诱导急性肝衰竭大鼠一氧化氮的影响

Effects of "Shengmai Powder" on Nitric Oxide of Acute Liver Failure Induced by Lipopolysaccharide

目的探讨生脉散对脂多糖(LPS)诱导急性肝衰竭大鼠一氧化氮的影响。方法采用D-氨基半乳糖腹腔注射法制作急性肝衰竭大鼠模型。生脉散灌胃2h及8h后,检测血清大肠杆菌LPS、肿瘤坏死因子(TNF-α)与一氧化氮(NO)、总一氧化氮合酶(zNOS)和诱导型一氧化氮合酶(iNOS)等水平。结果生麦散作用2h后可显著降低急性肝衰竭大鼠LPS、TNF-α和iNOS水平与对照组比较有统计学差异(P<0.001),LPS诱导8h后急性肝衰竭大鼠血清NO、zNOS和iNOS显著升高(P<0.001),生脉散能显著降低急性肝衰竭大鼠血清NO、iNOS水平(P<0.001),而对zNOS水平无明显影响。结论SD大鼠在急性肝衰竭状态下,存在严重内毒素血症,并引起一氧化氮水平变化的炎症级联反应,生脉散可通过调节一氧化氮水平起到治疗作用。

Objective To study the effects of ＂Shengmai Powder＂ on nitric oxide（NO） of acute liver failure induced by lipopolysaccharide in rats. Methods Duplicate the model of acute liver failure by D-galactoasmine. Two and 8 hours after treatment by ＂Shengmai Powder＂, the levels of serum LPS, TNF-α, NO, zNOS and iNOS were assayed respectively. Results Two hours after treatment, the levels of LPS, TNF-α and iNOS were obviously reduced（P 〈0. 001 ） ; 8-hour acute liver failure later, LPS, level of NO, zNOS and iNOS increased evidently（P 〈0. 001 ）, and ＂Shengmai Powder＂ could reduce the levels of NO and iNOS（ P 〈0. 001 ）. Conclusion In acute liver failure rats, serious endotoxemia develops and the level of NO becomes abnormal; ＂ Shengmai Powder＂ can regulate the level of NO.