大孔吸附树脂纯化补肾方中总黄酮的工艺研究

Purification of total flavones from Bushen Recipe with macroporous resin

目的:研究大孔吸附树脂吸附纯化补肾方(何首乌、淫羊藿苷、骨碎补等)总黄酮的特性及工艺条件。方法:选用D101、DA201和DM301树脂,以总黄酮、二苯乙烯苷、淫羊藿苷和柚皮苷为指标,综合考察3种大孔吸附树脂对补肾方中黄酮成分的吸附和解吸附性能,并以优选的树脂,进行静态动力学吸附曲线测定、动态泄漏曲线制备、洗脱曲线制备等研究。结果:选用DM301大孔吸附树脂,以0.25 BV(柱体积)/h速度上样,分别用4 BV蒸馏水洗除杂质,然后用70%乙醇4 BV洗脱纯化总黄酮,洗脱速度为2.4 V/h。结论:大孔吸附树脂用于补肾方总黄酮的提取纯化工艺获较好效果。

AIM： To study the absorption performance and purification process of total flavones from Bushen Recipe （Radix polygoni multiflori; Herba epimedii; Rhizoma drynariae; etc） with macroporous resin. METHODS： The contents of total flavones, icariin, tetrahydroxystilbene and naringin were worked as indexes, the ad- sorptive capacity of D101 ,DA201 and DM301 resins were investigated, and the static adsorption curve, dynamic leakage curve and elution curve were used for the selection of macroporous resin. RESULTS ： The elution parameters of selected DM301 consisted of the 0.25 BV/h feed rate solution ratio of 4 BV water to elute resin and 4 BV 70% alcohol to be collected as elution solvent, and the elution rate of 2.4 BV/h was established. CONCLUSION： Macroporous resin method is a better candidate method for purifying flavones in Bushen Recipe.