温热增强人肺癌细胞PLA-801D对多西紫杉醇的敏感性

Hyperthermia increases the sensitivity of human pulmonary carcinoma PLA-801D cells to Taxotere

目的探讨不同温热对多西紫杉醇增敏的规律及其机制。方法应用四氮唑蓝(MTT)快速比色法测定不同温度下的热疗组、单纯化疗组以及以不同序贯方式结合的热化疗组对人肺巨细胞癌细胞(PLA-801D)的生长抑制率;流式细胞仪测定42℃温热条件下各组细胞周期的变化。结果1)38～41℃的单纯热疗促进了肺癌细胞的生长(P<0.01);42～43℃的单纯热疗对肺癌细胞有抑制和杀伤作用(P<0.01);2)温热与药物同时作用效果最佳(P<0.001),且抑制率与温度的变化呈正相关(r=0.9920,P=0.0001);3)细胞周期分析发现热化同时组细胞各个周期增减的幅度最大。结论临床热疗不宜单独进行,需与化疗联合应用;一定范围内,温度越高热化疗的疗效越好。

Objective To investigate the sensitivity of hyperthermia at different temperatures to taxotere and its mechanism. Methods Growth inhibiting rates of human pulmonary carcinoma giant cells （PLA-801 D）in simple thermotherapy group, simple chemotherapy group, combined thermotherapy and chemotherapy group were determined by MTT colorimetric assay. Changes in cell cycle after treatment with hyperthermia at 42℃ and taxotere were analyzed by flow cytometry （FCM）. Results Hyperthermia at 42 -43℃ suppressed the proliferation of PLA-801D cells （ P 〈 0.01 ）. However, hyperthermia at 38 - 41 ℃ stimulated the proliferation of pulmonary carcinoma cells（ P 〈 0.01 ）. Hyperthermia in combination with drugs could achieve best results, and the inhibitory rate was positively correlated with the change of temperature （ r = 0. 992 0, P = 0. 000 1 ）. Cell cycle analysis showed that the amplitude of cell cycles changed greatly after combined thermotherapy and chemotherapy. Conclusion Simple hyperthermia should be applied in combination with chemotherapy. The higher the temperature of hyperthermia is, the better the effect of hyperthermia is, in the controlled range.