摘要
目的研究压疮大鼠缺血再灌注不同时间点骨骼肌的形态学变化。方法54只Wistar大鼠随机分为9组:对照组、缺血再灌注组(IR组):IR0h组、IR4h组、IR12h组、IR24h组、IR3d组、IR1周组、IR2周组、IR4周组,每组6只。观察受压部位骨骼肌的大体形态及HE染色光镜下观察其病理改变。结果实验组大鼠骨骼肌结构发生进行性损伤,肌纤维出现溶解、断裂、空泡变、水肿、骨骼肌横纹消失、毛细血管扩张充血,间质水肿等病理改变。在施压后24h损伤最严重,施压后4周,仍未完全修复。结论压疮形成过程中受压部位骨骼肌的损伤是一个渐进的过程,在受压后不同时间损伤的表现不同。
Objective To investigate the histological changes in skeletal muscle of rats with pressure ulcer. Methods 54 Wistar rats were randomly divided into 9 groups: nonischemia control group and ischemiareperfusion groups(IR group). IR0h group, IR4h group, IR12h group, IR24h group, IR3day group, IRlweek group, IR2week group, IR4week group,each group had six rats.The morphological change of skeletal muscle at different time points were tested by H-E staining.Results There was progressive impairment of skeletal muscle,such as dissolve, rupture, edema, disappeared transverse striation、 hemangiectasis and congestion interstitial edema. The skeletal muscle injury reached the peak at IR24h,then relieved gradually,but couldn't recover till IR4week.Conclusions The injury of chushed skeletal muscle was a dynamic developing course during the process of pressure ulcer.The pathologic change at erery time point was different.
出处
《中国血液流变学杂志》
CAS
2009年第2期192-194,共3页
Chinese Journal of Hemorheology
关键词
压疮
缺血再灌注
骨骼肌
形态学
pressure ulcer
ischemia reperfusion
morphologic