Calretinin在先天性巨结肠中的表达及其意义

Expressions of calretinin and its significance in the colon of patients with Hirschsprung's disease (HD)

目的探讨钙视网膜蛋白(calretinin,CR)在先天性巨结肠(HD)中的表达及其意义。方法对18例HD扩张段结肠和痉挛段结肠行免疫组织化学染色。利用图文分析系统观察染色表达情况。结果CR在HD患儿扩张段肠壁神经节细胞及神经纤维内表达,免疫反应强阳性,CR蛋白表达稳定。而HD患儿痉挛段CR蛋白表达明显减弱,近乎缺失。结论CR可以作为HD诊断的一个辅助指标。

Objective To investigate the expressions of calretinin and its significance in the colon of patients with Hirschsprung＇s disease. Methods We examined the expressions of CR protein in 18 HD aganglionic segments and 18 ganglionic segments by means of immunohistochemistry. Results There was a sinigicant difference in the expressions of Calretinin immunohistochemistry between HD ecstatic group and HD spastic group （P〈0.01）. CR was almost not expressed in spastic segments of HD, whereas in ecstatic segments of HD both ganglion cells and nerve fibers were immunopositive. Conclusion The absence of calretinin expression in spastic segments of HD may serve as a diagnostic aid in identifying aganglion segments in HD.