海龙及其常见伪品的RAPD鉴别

Identification on Syngnathus and its adulterants with random amplified polymorphic DNA

目的:提供一种鉴别中药材海龙及其伪品的方法。方法:采用随机引物扩增DNA技术,对拟海龙、刁海龙、尖海龙、粗吻海龙、海蠋鱼、宝珈海龙进行基于Nei&Li's相似系数的UPGMA聚类分析,并构建其鉴别模式。结果:从18条随机引物中筛选到LJ04,LJ09,LJ16,LJ19 4条引物能扩增出稳定可靠的条带。基于以上4条随机引物构建的聚类图能较好的从属水平区分各海龙样品,且与样品外观分析结果一致。基于LJ09,LJ19 2条引物的扩增结果构建的6种海龙的鉴别模式可以将海龙正品逐一鉴别出来。结论:随机引物扩增DNA技术能较好地鉴别海龙及其伪品。

Objective： To study the identification method of Syngnathus and its adulterants. Method ： Random amplified poly- morphic DNA （RAPD） was used to construct a dendrogram by UPGMA method based on Nei ＆ Li＇s coefficient and a genetic affinity pattern for Syngnathus acus, Solenognathus hardwickii, Syngnathoides biaculeatus, Trach^hamphus serratus, Halicampus koilomat- odon, Microphis boaja. Result： Four primers, LJ04, LJ09, LJI6 and LJ19, from 18 random primers were used in the dendrogram which can differentiate Syngnathus in genus level and showed a great consistence with the appearance identification. The genetic affinity pattern based on primers LJ09 and LJ19 could be used to identify Syngnathus from its adulterants. Conclusion： RAPD is suitable to identify Syngnathus and its adulterants.