大粒车前子中苯乙醇苷类化合物对树突状细胞的调控研究

Effects of phenylethanoid glycosides from seeds of Plantago asiatica on maturation of dendritic cells

目的:探讨大粒车前子中苯乙醇苷类化合物对小鼠骨髓来源树突状细胞(DCs)功能调节的影响,为进一步阐明大粒车前子的免疫调节活性提供依据。方法:采用细胞因子诱导法,从Balb/cj小鼠骨髓细胞贴壁分离获得单核细胞,加入含10%胎牛血清、10μg.L-1重组小鼠粒细胞巨噬细胞集落刺激因子(rmGM-CSF)及重组小鼠白细胞介素-4(rmIL-4)的RPMI1640完全培养基培养,在培养第6天,实验组加入车前子苯乙醇苷类化合物(10,50,100 mg.L-1),对照组加入RPMI 1640或LPS(1 mg.L-1),流式细胞仪检测DCs表面分子CD11c,CD86,MHC II和CD80的表达以及各组DCs吞噬功能的变化。结果:车前子毛蕊花苷和异毛蕊花苷能够提高DCs表面分子CD11c,CD86,MHC II和CD80的表达;空白组DCs的吞噬功能很强(45.39%),车前子毛蕊花苷和异毛蕊花苷组DCs吞噬功能都明显下降(分别为37.27%,30.40%,33.45%和40.15%,37.59%,31.07%)。结论:车前子苯乙醇苷类化合物能促进小鼠骨髓来源的DCs表型及功能的成熟。

Objective： To elucidate the immunomodulatory mechanism of phenylethanoid glycosides from the seeds of Plantago asiatica by testing its effects on the maturing of murine bone marrow derived dendritic cells （DCs）. Method： Monocytes generated from bone marrow of Balb/cj mouse were cultured for 6 days in complete RPMI 1640 medium containing 10% FBS, rmGM-CSF and rmIL-4. 50 mg ~ L-1 acteoside or isoacteoside was added to cells on day 6 of culture for 24 h. The surface molecules expression level of DCs and their phagocytose ability were analysis by flow cytometry. Result： Both acteoside and isoacteoside could increase the ex- pression of CD11 c, CD86, MHC II and CDS0 on DCs surface. The ability of unstimulated DCs to uptake FITC-dextran was higher than that of phenylethanoid glycosides or LPS treated DCs. Conclusion： Both acteoside and isoacteoside could induce maturation of murine dendritic cells.