丹参毛状根对大鼠离体心脏缺血再灌注损伤的保护作用

Protection on myocardial ischemia and reperfusion injury by Salvia miltiorrhiza hairy root in isolated Langendorff rat hearts

目的:观察丹参毛状根对离体大鼠心肌缺血再灌注损伤的保护作用。方法:采用Langendorff离体心脏恒压灌流方法,以0.5 mL.min-1的灌流速度,生药质量浓度分别为14,28,56 mg.L-1的丹参毛状根,与k-H灌流液同时灌流心脏10min,然后全心停灌30 min,复灌60 min。结果:离体实验发现,丹参毛状根能够增加冠脉流量,不但降低心律失常的发生率,而且推迟心律失常的发生时间,缩短持续时间和恢复时间,减低LDH,MDA的释放,增加SOD活性。结论:丹参毛状根对大鼠离体心脏缺血再灌注损伤具有一定的保护作用;与丹参药材相比无显著性差异。

Objective： Protection by Salvia miltiorrhiza hairy root on myocardial ischemia and reperfusion injury in isolated Langendorff rat hearts was observed. Method： Isolated rat hearts were perfused with 14, 28, 56 mg · L-1 for 10 min followed by a 10 min washout period before the induction of 30 min norrnothermic global ischemia and 60 min of reperfusion. Result： Our results showed that S. miltiorrhiza hairy root reduced the incidence of reperfusion-induced VF and VT and delayed the onset time of VF and VT. The increased aortic flow and content of SOD with decreased release of LDH and MDA were found. Conclusion： Our results show that S. miltiorrhiza hairy root can prevent postischmic abnormalities in reperfusion arrhythmias, aortic flow and myocardium metabolism, which was similar to actions of S. miltiorrhiza.