D-半乳糖大鼠衰老模型的评价

The Assessment of the D-galactose Rat Aging Model

目的对D-半乳糖衰老模型大鼠进行应激、智力、免疫以及皮肤结构的评价。方法1月龄健康SD大白鼠50只用随机数字表法分为两组:衰老模型组25只:注射定量的D-半乳糖促使其衰老;年轻对照组25只:SD大鼠空白对照。分别测定两组应激、智力、耐缺氧、免疫以及真皮厚度等指标,并进行比较。结果衰老模型组的抗疲劳能力低于年轻对照组(P<0.001),Y迷宫智力测验及跳台智力测验的错误反应率均高于年轻对照组(P<0.001),T淋巴细胞转化率和溶血素水平均低于年轻对照组(P<0.001),耐缺氧时间短于年轻对照组(P=0.002),真皮厚度低于年轻对照组(P<0.001)。结论D-半乳糖注射后的衰老模型大鼠,抗应激能力低下,免疫功能降低,学习记忆力衰退,真皮厚度变薄。

Objective To assess the resistance various stresses,the intelligence,the immunity function and the dermal thickness in the rat aging model. Methods 50 SD rats of 30 day-old were divided into two groups at ramdom. The aging model group included 25 rats by injection of quantitive D-galactose to establish aging model. The young group included 25 rats as controlled. The resistance against stress ,the intelligence, the immunity and the dermal thickness were detected and compared. Results Compared to the young group, the resistance abilities of aging model to fatigue and the normal hypoxia tolerance decreased （ P 〈 0.001 ） ; the wrong reactive rates of ＂Y＂ Maze and Step Down Test increased （P 〈 0.001 ） ;T lymphocyte transforming rate and the level of hemolysin decreased （P 〈 0.001 ） ;the dermal thickness of aging model decreased （P 〈 0. 001 ）. Conclusion The aging model of rat establish by injection of D-galactese charaeterized by the decreased of abilities of resistance various stress, immunity, memory and the dermal thickness.